Kinomics hat nutzbringende, einzigartige
Erweiterungen für Affinitäts-Biosensoren definiert.
Kinomics MSK® Application Note
Affinogram created by subtraction
of reference flow cell 1 from active flow cell
2 (which was loaded only once before at
560 RU of captured antibody ligand), recorded
at a flow rate of 100 µL
using a Biacore®3000 instrument. Solid arrows
indicate the start of 2 min injections of protein
analyte (the first was a blank = buffer; each
other step doubled in concentration). Each analyte
injection was followed by a 2.5 min buffer wash
(dissociation), indicated by dashed arrows. The
association and dissociation steps were fitted
and evaluated by means of the Kinomics MSK® program,
yielding kinetics constants comparative to those
found by the traditional sequential approach.
- Note the time scale of the entire run of just
approx. 45 min in comparison with repeated intermediate
ligand re-captures (1.5 to 3 h) or a long-term
equilibration titration (5 to 10 h)!
Acknowledgement: The experimental data were kindly
provided by Dr Martin Lanzendoerfer and Mrs. Birgit
Essig, Roche Diagnostics GmbH, Penzberg, Germany.
Biacore®3000 is a trademark
of Biacore AB, Uppsala, Sweden.