Kinomics MSK® Technical Note Overview
The upper figure shows MSK® variants,
resulting in different response-over-time affinograms.
Solid arrows indicate an increase of the analyte
concentration, dashed arrows denote a concentration
decrease. Most time-saving, a revolving incremental
increase can be applied (a). Common biosensor
injectors permit alternating association / dissociation
phases (c); an intermediate assay is shown in
As commonly known, low analyte concentrations
frequently suffer from mass-transport limitation
(MTL) that often is hidden in further
data analysis. In order to solve this problem,
a new graph was defined by Kinomics. Sophisticated
initial rate analysis at each change point (solid
/ dashed arrows) yields transformed initial rates*,
which (when plotted versus starting response)
resolves important kinetics parameters in a single
plot (d) - while detecting and considering MTL
(red dots/circles in d) in a much more sensitive
way than any other kinetics plot did so far.
Most importantly (and beyond other advantages),
· significantly detects mass-transport
limitations and rebinding effects and
· permits the determination of the association
rate constant (kass) and the
from a single plot.
The quantities plotted in this graph are orally
quite long expressions*. Hence, in accordance
with early-stage users of the Kinomics MSK® program,
this plot has been coined "Trutnau plot"
following the name of its inventor. - Three further
plots introduced in the MSK® approach ("northeast",
"southwest", and "southeast"
located in the arrangement of the six plots shown
in the MSK® Product Note) have also been applied
this way for the first time in kinetics utilizing
*The concentration-normalized, net-initial association
rate (mNIAR, dots in d) and the corresponding
net-final association rate (mNFAR, circles
in d), plotted versus the actual starting response.
Online publiziert 10.03.2005
Kinomics MSK® Technical Note